Human Anti-TREM2 Recombinant Antibody (TAB-168CQ)
CAT#: TAB-168CQ
This product is a recombinant human antibody that recognizes TREM-2 (Triggering receptor expressed on myeloid cells-2). The antibody was purified by affinity chromatography. It can be used for ELISA, FC, WB, FuncS and other applications.
Tested Data
Gene Expression
SDS-PAGE
Figure 1 Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) in SDS-PAGE
SDS-PAGE analysis of TAB-168CQ in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) in SEC-HPLC
The purity of TAB-168CQ was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
WB
Figure 3 Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) in WB
Western blot analysis of TAB-168CQ was performed by loading Human TREM-2 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-168CQ and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (1 μg)
DB
Figure 4 Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) in DB
Dot Blot analysis of TAB-168CQ was performed by coating with Human TREM-2 Protein (His Tag).
TAB-168CQ incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
ELISA
Figure 5 Human Anti-TREM2 Recombinant Antibody (TAB-168CQ) in ELISA
ELISA analysis of TAB-168CQ was performed by coating with Human TREM-2 Protein (His Tag). Then blocked with BSA, and incubated with TAB-168CQ. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Normal Tissue
Figure 1 IHC staining of human gallbladder
Immunohistochemical staining of human gallbladder shows strong membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10917/26581_A_6_4_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10917/26581_B_8_5.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10917/26581_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10917/26581_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (hTCEpi, THP-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000095970-TREM2
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Specifications
- Immunogen
- The extracellular domain of TREM2
- Host Species
- Human
- Derivation
- Human synthetic yeast libraries
- Type
- Human IgG
- Specificity
- Human
- Species Reactivity
- Human, Mouse
- Applications
- ELISA, FC, WB, FuncS
- Related Disease
- Alzheimer's disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Customer Review
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Good for Western Blotting
I purchased this antibody for detecting TREM2 protein levels in cell lysates using Western blotting. The antibody yielded a clear band at the expected molecular weight for human TREM2, and there were minimal non-specific bands, which is crucial for accurate quantification. I appreciated the high purity of the antibody as indicated in the product information, which likely contributed to the clean results I obtained. I would recommend this antibody for Western blot applications targeting TREM2.
Effective Flow Cytometry Antibody
I utilized this Anti-TREM2 antibody for flow cytometric analysis of TREM2 expression on the surface of primary human microglia. After optimizing the staining protocol, I was able to clearly distinguish TREM2-positive and negative populations. The antibody showed good specificity and provided a reliable method for quantifying TREM2 expression levels in my cell culture model. This antibody is a suitable choice for researchers employing flow cytometry to study TREM2 on myeloid cells.
Q&As
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I need to perform Western blotting to assess TREM2 expression levels in cell lysates. What is the expected molecular weight of the human TREM2 protein that this antibody will recognize under reducing conditions?
A: The mature human TREM2 protein is a glycoprotein with a reported molecular weight that can vary due to glycosylation. Based on literature, the molecular weight typically ranges from approximately 25 kDa to 35 kDa. When performing Western blotting, you should expect to see a band within this range. Ensure your protein samples are properly prepared and consider using a positive control to confirm the antibody's specificity and the expected band size.
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I'm interested in using flow cytometry to analyze TREM2 expression on the surface of microglia. Has this antibody been tested and validated for flow cytometry on live or fixed cells, and what specific fixation and permeabilization protocols are recommended?
A: The product datasheet indicates that flow cytometry (FC) is a validated application for this antibody. However, specific protocols for live versus fixed cell staining, as well as recommended fixation and permeabilization reagents, are usually dependent on the experimental design and cell type. I would recommend contacting the technical support team at Creative Biolabs for detailed protocols or any available internal validation data for flow cytometry using this specific antibody (TAB-168CQ) on microglia or similar cell types.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-168CQ, RRID: AB_3111846)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-169CQ | Human Anti-TREM2 Recombinant Antibody (TAB-169CQ) | ELISA, FC, WB, FuncS | Human IgG |
| TAB-168CQ-S(P) | Human Anti-TREM2 Recombinant Antibody; scFv Fragment (TAB-168CQ-S(P)) | ELISA, FC, WB, FuncS | Human scFv |
| TAB-169CQ-S(P) | Human Anti-TREM2 Recombinant Antibody; scFv Fragment (TAB-169CQ-S(P)) | ELISA, FC, WB, FuncS | Human scFv |
| TAB-168CQ-F(E) | Human Anti-TREM2 Recombinant Antibody; Fab Fragment (TAB-168CQ-F(E)) | ELISA, FC, WB, FuncS | Human Fab |
| TAB-169CQ-F(E) | Human Anti-TREM2 Recombinant Antibody; Fab Fragment (TAB-169CQ-F(E)) | ELISA, FC, WB, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0152-CN-S(P) | Human Anti-TREM2 Recombinant Antibody (clone Pil.02.002); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-0153-CN-S(P) | Human Anti-TREM2 Recombinant Antibody (clone Pil.02.005); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-0154-CN-S(P) | Mouse Anti-TREM2 Recombinant Antibody; scFv Fragment (HPAB-0154-CN-S(P)) | WB, ELISA, FC, IHC, IF, IP | Mouse scFv |
| HPAB-0155-CN-S(P) | Mouse Anti-TREM2 Recombinant Antibody; scFv Fragment (HPAB-0155-CN-S(P)) | WB, ELISA, FC, IHC, IF, IP | Mouse scFv |
| HPAB-0156-CN-S(P) | Mouse Anti-TREM2 Recombinant Antibody; scFv Fragment (HPAB-0156-CN-S(P)) | WB, ELISA, FC, IHC, IF, IP | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0152-CN-F(E) | Human Anti-TREM2 Recombinant Antibody (clone Pil.02.002); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-0153-CN-F(E) | Human Anti-TREM2 Recombinant Antibody (clone Pil.02.005); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-0154-CN-F(E) | Mouse Anti-TREM2 Recombinant Antibody; Fab Fragment (HPAB-0154-CN-F(E)) | WB, ELISA, FC, IHC, IF, IP | Mouse Fab |
| HPAB-0155-CN-F(E) | Mouse Anti-TREM2 Recombinant Antibody; Fab Fragment (HPAB-0155-CN-F(E)) | WB, ELISA, FC, IHC, IF, IP | Mouse Fab |
| HPAB-0156-CN-F(E) | Mouse Anti-TREM2 Recombinant Antibody; Fab Fragment (HPAB-0156-CN-F(E)) | WB, ELISA, FC, IHC, IF, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-3341LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3341LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
| HPAB-3342LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3342LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
| HPAB-3343LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3343LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
| HPAB-3344LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3344LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
| HPAB-3345LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3345LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY58 | Mouse Anti-TREM2 Agonistic Antibody (clone al002c) | Agonistic assays, DB, ICC, IP, SPR, IF | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY262 | AbPlus™ Anti-TREM2 Magnetic Beads (VS-0424-XY262) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2348 | Anti-TREM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7507 | Anti-Rat TREM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7508 | Anti-Mouse TREM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7506 | Anti-Human TREM2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC228 | Recombinant Anti-TREM2 Vesicular Antibody, EV Displayed (VS-0425-YC228) | ELISA, FC, Cell-uptake |
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